3ZCR
Rabbit muscle glycogen phosphorylase b in complex with N-(4-tert- butyl-benzoyl)-N-beta-D-glucopyranosyl urea determined at 2.07 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2005-05-14 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.531, 128.531, 116.687 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.550 - 2.070 |
| R-factor | 0.18034 |
| Rwork | 0.179 |
| R-free | 0.20836 |
| Structure solution method | MIR |
| Starting model (for MR) | 2qnb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.998 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.110 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.070 | 0.400 |
| Number of reflections | 57679 | |
| <I/σ(I)> | 18.8 | 5.7 |
| Completeness [%] | 96.0 | 93.1 |
| Redundancy | 6.5 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | pH 6.8 |
