3UTY

Crystal structure of bacteriorhodopsin mutant P50A/T46A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97920
Spacegroup name	P 1 21 1
Unit cell lengths	45.781, 116.463, 57.083
Unit cell angles	90.00, 112.55, 90.00

Refinement procedure

Resolution	90.000 - 2.370
R-factor	0.2154
Rwork	0.214
R-free	0.23880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	2.016
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.000	2.450
High resolution limit [Å]	2.370	2.370
Number of reflections	21876
Completeness [%]	100.0	96.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BICELLES, VAPOR DIFFUSION, HANGING DROP	4	310	0.65M sodium phosphate, 0.95% triethylene glycerol, 0.008M 1,6-hexanediol, 4.3% DMPC, 1.5% CHAPSO, BICELLES, VAPOR DIFFUSION, HANGING DROP, temperature 310K