3T9L

Structure of N-terminal DUSP-UBL domains of human USP15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9763
Spacegroup name	P 31 2 1
Unit cell lengths	97.601, 97.601, 69.398
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.262 - 1.500
R-factor	0.1585
Rwork	0.156
R-free	0.17780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 1W6V and 3JYU
RMSD bond length	0.005
RMSD bond angle	0.934
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	84.525	84.525	1.560
High resolution limit [Å]	1.479	4.680	1.480
Rmerge		0.068	0.292
Total number of observations		36566	82766
Number of reflections	63890
<I/σ(I)>	22.5	8.5	2.6
Completeness [%]	99.7	100	97.9
Redundancy	10.5	16.8	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	298	2M ammonium sulphate, 100 mM Bis-Tris-Cl, pH 5.5, vapor diffusion, temperature 298K