3CN6
Crystal structure of the Spinach Aquaporin SoPIP2;1 S274E mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Detector technology | CCD |
| Collection date | 2007-09-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.931 |
| Spacegroup name | I 4 |
| Unit cell lengths | 89.558, 89.558, 189.271 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.860 - 2.950 |
| R-factor | 0.19736 |
| Rwork | 0.196 |
| R-free | 0.22985 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.052 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.860 | 3.110 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Number of reflections | 15560 | |
| Completeness [%] | 100.0 | |
| Redundancy | 4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 280 | Reservoir: 0.1M Tris, 0.2M NaCl, 24% PEG400. Reservoir mixed 1:4 with 0.1M CdCl2, and then mixed 1:1 with protein, centrifuged briefly and supernatant used for crystallization, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 280K |
