315D
CRYSTAL STRUCTURE OF AN ALTERNATING OCTAMER R(GUAUGUA)D(C) WITH ADJACENT G-U WOBBLE PAIRS
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS |
| Spacegroup name | H 3 |
| 格子定数 [Å] | 39.710, 39.710, 68.150 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 10.000 - 1.380 |
| R因子 | 0.176 |
| Rwork | 0.176 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AHH071 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.015 |
| 結合角の平均二乗偏差(RMSD) [度] | 3.300 |
| Data reduction software | XENGEN (V. 2.0) |
| Refinement software | X-PLOR (3.0) |
Quality characteristics
| Overall | |
| 分解能 [Å] (高) | 1.380 |
| Rmerge_l_obs | 0.035 * |
| 独立反射数 | 5744 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 18 * | pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 291.00K |
結晶化試薬
| ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | [CO(NH3)6]3+ | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | RNA | 1 (mM) | |
| 2 | 1 | drop | cobaltic hexammine chloride | 0.5 (mM) | |
| 3 | 1 | drop | sodium cacodylate | 40 (mM) | |
| 4 | 1 | drop | MPD | 10 (%(v/v)) | |
| 5 | 1 | reservoir | MPD | 60 (%(v/v)) |
