Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 153 |
| Detector technology | DIFFRACTOMETER |
| Detector | RIGAKU AFC-5R |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 26.450, 34.660, 32.170 |
| 格子定数 [度] | 90.00, 113.40, 90.00 |
精密化法
| 残基 | 8.000 - 2.600 |
| R因子 | 0.214 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.023 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.031 |
| Refinement software | NUCLSQ |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 3.600 | |
| 分解能 [Å] (高) | 2.600 | 2.600 |
| 独立反射数 | 1768 | |
| 完全性 [%] | 75 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | VAPOR DIFFUSION, SITTING DROP, temperature 291K |
結晶化試薬
| ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MG ACETATE | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | DNA | 1 (mM) | |
| 2 | 1 | drop | magnesium acetate | 30 (mM) | |
| 3 | 1 | drop | spermine | 1 (M) | |
| 4 | 1 | drop | MPD | 10 (%(v/v)) | |
| 5 | 1 | reservoir | MPD | 15 (%) |
