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2ZXQ

Crystal structure of endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum (EngBF)

Experimental procedure
Experimental methodMAD
Source typeSYNCHROTRON
Source detailsPHOTON FACTORY BEAMLINE BL-6A
Synchrotron sitePhoton Factory
BeamlineBL-6A
Temperature [K]100
Detector technologyCCD
Collection date2008-02-17
DetectorADSC QUANTUM 4
Wavelength(s)0.96787, 0.97923, 0.97939, 0.96416
Spacegroup nameP 65
Unit cell lengths192.267, 192.267, 123.017
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution36.490 - 2.000
R-factor0.17324
Rwork0.172
R-free0.19564
Structure solution methodMAD
RMSD bond length0.014
RMSD bond angle1.408
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareSOLVE
Refinement softwareREFMAC (5.2.0019)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.070
High resolution limit [Å]2.0002.000
Number of reflections172447
<I/σ(I)>27.23.5
Completeness [%]99.199.8
Redundancy7.26.5
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.92930.1M MES-NaOH, 3% PEG 20000, 25% MPD, 0.2M NaCl, 0.01M MnCl2, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K

217705

건을2024-03-27부터공개중

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