2ZOV
Structure of the periplasmic domain of MotB from Salmonella (crystal form I)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979101,0.979397,0.9730,0.9860 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 99.740, 99.740, 43.960 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.650 - 2.000 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.26500 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 17162 | |
| <I/σ(I)> | 22.1 | 8 |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 9.7 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 6% PEG 4000, 0.1M Acetate, 0.22M Zn(OAc)2, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
