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2YSS

Crystal structure of Humanized HYHEL-10 FV mutant(HQ39KW47Y)-HEN lysozyme complex

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsPHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron sitePhoton Factory
BeamlineAR-NW12A
Temperature [K]95
Detector technologyCCD
Collection date2004-11-09
DetectorADSC QUANTUM 210
Wavelength(s)1.0000
Spacegroup nameP 21 21 21
Unit cell lengths62.302, 73.933, 75.640
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution19.990 - 2.400
R-factor0.218
Rwork0.218
R-free0.26500
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2eiz
RMSD bond length0.007
RMSD bond angle1.300
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareMOLREP
Refinement softwareCNS (1.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.490
High resolution limit [Å]2.4002.400
Rmerge0.1380.344
Number of reflections14041
Completeness [%]99.6
Redundancy6.56.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.429328-31% PEG MME 550, 0.01M zinc sulfate, 0.1M MES, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

217705

数据于2024-03-27公开中

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