2Y5W
Crystal structure of Drosophila melanogaster kinesin-1 motor domain dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2009-12-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.890, 89.180, 138.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.740 - 2.700 |
| R-factor | 0.21384 |
| Rwork | 0.210 |
| R-free | 0.28883 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bg2 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.829 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.840 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.080 | 0.400 |
| Number of reflections | 35890 | |
| <I/σ(I)> | 12 | 3.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | 24 % POLYETHYLENE GLYCOL-3350, 0.15 M AMMONIUM SULFATE, AND 0.1 M HEPES SODIUM PH 7.8 |
