2Y1N
Structure of c-Cbl-ZAP-70 peptide complex
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 |
| 格子定数 [Å] | 93.570, 93.570, 189.599 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 45.423 - 1.999 |
| R因子 | 0.159 |
| Rwork | 0.158 |
| R-free | 0.18040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cbl |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.008 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.142 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.070 |
| 分解能 [Å] (高) | 2.000 | 2.000 |
| Rmerge_l_obs | 0.080 | 0.520 |
| 独立反射数 | 63238 | |
| <I/σ(I)> | 30.3 | 2.5 |
| 完全性 [%] | 99.9 | 99.7 |
| 冗長性 | 3.8 | 3.4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 18-20% (W/V) PEG 3350, 0.2 M AMMONIUM FORMATE |
