2XTE
Structure of the TBL1 tetramerisation domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-22 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.020, 150.190, 164.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 111.100 - 3.900 |
R-factor | 0.273 |
Rwork | 0.273 |
R-free | 0.30200 |
Structure solution method | MIR |
Starting model (for MR) | 2xtc |
RMSD bond length | 0.011 |
RMSD bond angle | 1.240 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 111.100 | 4.110 |
High resolution limit [Å] | 3.900 | 3.900 |
Rmerge | 0.110 | 0.320 |
Number of reflections | 19503 | |
<I/σ(I)> | 9.1 | 4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.5 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 0.1 M MES PH6.5, 2.0 M NACL, 0.175 M SODIUM ACETATE, 19 % GLYCEROL |