2X61
Crystal structure of the sialyltransferase CST-II in complex with trisaccharide acceptor and CMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-10 |
Detector | MARRESEARCH |
Spacegroup name | P 4 |
Unit cell lengths | 117.665, 117.665, 46.604 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.950 |
R-factor | 0.16553 |
Rwork | 0.164 |
R-free | 0.19752 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ro8 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.068 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.080 | 0.370 |
Number of reflections | 46782 | |
<I/σ(I)> | 38.3 | 5 |
Completeness [%] | 99.6 | 96.9 |
Redundancy | 6.3 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 100 MM HEPES5, PH 7.5, 8% (W/V) POLYETHYLENE GLYCOL 6000, AND 5% (V/V) 2-METHYL-2, 4-PENTANEDIOL |