2WXB
Acetylglutamate kinase from Escherichia coli free of substrates
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-02-24 |
Detector | MARRESEARCH |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 78.696, 78.696, 277.743 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.950 - 2.000 |
R-factor | 0.216 |
Rwork | 0.214 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gs5 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.203 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.110 |
High resolution limit [Å] | 1.950 | 2.000 |
Rmerge | 0.050 | 0.280 |
Number of reflections | 35514 | |
<I/σ(I)> | 11.8 | 2.7 |
Completeness [%] | 99.8 | 100 |
Redundancy | 6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | 0.1 M SODIUM CITRATE PH 5.6, 26-32% PEG 4000 AND 0.1-0.3 M AMMONIUM ACETATE |