2WIP
STRUCTURE OF CDK2-CYCLIN A COMPLEXED WITH 8-ANILINO-1-METHYL-4,5-DIHYDRO- 1H-PYRAZOLO[4,3-H] QUINAZOLINE-3-CARBOXYLIC ACID
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-04-02 |
| Detector | RIGAKU R-AXIS IV |
| Spacegroup name | P 62 2 2 |
| 格子定数 [Å] | 184.185, 184.185, 215.005 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 28.400 - 2.800 |
| R因子 | 0.221 |
| Rwork | 0.221 |
| R-free | 0.25800 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.006 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (NULL) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.000 | 2.850 |
| 分解能 [Å] (高) | 2.800 | 2.800 |
| Rmerge_l_obs | 0.080 | 0.300 |
| 独立反射数 | 50354 | |
| <I/σ(I)> | 23.8 | 6.2 |
| 完全性 [%] | 94.1 | 95 |
| 冗長性 | 6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 20% AMMONIUM SULPHATE, 1M KCL, 40MM HEPES PH 7 |
