2WIA
Crystal Structures of the N-terminal Intracellular Domain of FeoB from Klebsiella Pneumoniae in Apo Form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 63 |
Unit cell lengths | 84.204, 84.204, 122.457 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.130 - 2.450 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | STRUCTURE OF FEOB IN GMPPNP STATE |
RMSD bond length | 0.031 |
RMSD bond angle | 2.700 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.130 | 2.540 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.100 | 0.280 |
Number of reflections | 18109 | |
<I/σ(I)> | 18.9 | 6.1 |
Completeness [%] | 99.7 | 100 |
Redundancy | 4.6 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 100 MM TRIS-HCL PH 8.5, 30% PEG4000, 200 MM MGCL2 |