2VS7
The crystal structure of I-DmoI in complex with DNA and Ca
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Wavelength(s) | 0.9, 1.5414 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 106.988, 70.587, 106.848 |
Unit cell angles | 90.00, 119.75, 90.00 |
Refinement procedure
Resolution | 25.760 - 2.050 |
R-factor | 0.189 |
Rwork | 0.187 |
R-free | 0.22800 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.008 |
RMSD bond angle | 1.384 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.060 | 0.450 |
Number of reflections | 87351 | |
<I/σ(I)> | 14.9 | 1 |
Completeness [%] | 95.4 | 72.5 |
Redundancy | 3.6 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6% PEG 4000, 0.07M SODIUM ACETATE, 30% GLYCEROL |