2VQ4
Carbohydrate-binding of the starch binding domain of Rhizopus oryzae glucoamylase in complex with beta-cyclodextrin and maltoheptaose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 273 |
Detector technology | CCD |
Collection date | 2008-02-01 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 26.635, 33.894, 93.389 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.250 |
R-factor | 0.147 |
Rwork | 0.146 |
R-free | 0.16900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v8l |
RMSD bond length | 0.010 |
RMSD bond angle | 1.382 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.290 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 0.070 | 0.290 |
Number of reflections | 23978 | |
<I/σ(I)> | 31.5 | 6.7 |
Completeness [%] | 98.5 | 93.9 |
Redundancy | 11.6 | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 25% PEG4000 |