2VFV
Alditol Oxidase from Streptomyces coelicolor A3(2): Complex with Sulphite
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Collection date | 2007-03-15 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 105.561, 68.455, 57.965 |
Unit cell angles | 90.00, 95.19, 90.00 |
Refinement procedure
Resolution | 19.510 - 1.720 |
R-factor | 0.153 |
Rwork | 0.152 |
R-free | 0.18400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vfr |
RMSD bond length | 0.015 |
RMSD bond angle | 1.730 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 |
Phasing software | CCP4 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.900 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.040 | 0.090 |
Number of reflections | 43108 | |
<I/σ(I)> | 24.9 | 10.9 |
Completeness [%] | 98.5 | 97.1 |
Redundancy | 3.1 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | pH 6.5 |