2V5R
Structural basis for Dscam isoform specificity
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-09 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 277.781, 70.548, 72.771 |
| 格子定数 [度] | 90.00, 105.13, 90.00 |
精密化法
| 残基 | 20.000 - 3.000 |
| Rwork | 0.271 |
| R-free | 0.30300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v5m |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 20.000 | 3.200 |
| 分解能 [Å] (高) | 3.000 | 3.000 |
| Rmerge_l_obs | 0.100 | 0.230 |
| 独立反射数 | 53580 | |
| <I/σ(I)> | 10.9 | 1.9 |
| 完全性 [%] | 86.0 | 55.5 |
| 冗長性 | 2.8 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 10 % PEG 8000, 1MM SPERMIDINE, 0.1 M TRISHCL PH 8.5 |
