2UZP
Crystal structure of the C2 domain of human protein kinase C gamma.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-18 |
Detector | MARRESEARCH |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 138.503, 138.503, 68.358 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.172 |
Rwork | 0.171 |
R-free | 0.20500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dsy |
RMSD bond length | 0.013 |
RMSD bond angle | 1.313 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.080 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.100 | 0.700 |
Number of reflections | 45478 | |
<I/σ(I)> | 15.7 | 3.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.2 | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 1.8M AMMONIUM SULPHATE, 15MM COBALT CHLORIDE, 0.1M MES PH6.5, pH 6.50 |