2UZK
Crystal structure of the human FOXO3a-DBD bound to DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 61 |
Unit cell lengths | 41.964, 41.964, 354.817 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.130 - 2.700 |
R-factor | 0.242 |
Rwork | 0.242 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2c6y |
RMSD bond length | 0.012 |
RMSD bond angle | 2.600 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.050 | 0.420 |
Number of reflections | 9491 | |
<I/σ(I)> | 40.2 | 2.5 |
Completeness [%] | 97.1 | 97.3 |
Redundancy | 4.2 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |