2RJH
Crystal structure of biosynthetic alaine racemase in D-cycloserine-bound form from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-11-16 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 6 |
Unit cell lengths | 147.713, 147.713, 163.724 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 2.400 |
Rwork | 0.199 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rcq |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 164.399 | 40.930 | 2.530 |
High resolution limit [Å] | 2.400 | 7.590 | 2.400 |
Rmerge | 0.121 | 0.037 | 0.395 |
Total number of observations | 28683 | 88892 | |
Number of reflections | 78993 | ||
<I/σ(I)> | 6 | 17 | 1.9 |
Completeness [%] | 99.9 | 99.3 | 99.7 |
Redundancy | 9.7 | 11.2 | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.1M HEPES, 1.6M ammonium sulfate, pH7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |