2RJF

Crystal structure of L3MBTL1 in complex with H4K20Me2 (residues 12-30), orthorhombic form I

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ DW
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-01-10
Detector	RIGAKU RAXIS
Wavelength(s)	1.54000
Spacegroup name	P 21 21 21
格子定数 [Å]	90.458, 117.809, 132.316
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	39.650 - 2.050
R因子	0.18142
Rwork	0.179
R-free	0.22533
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pqw
結合長の平均二乗偏差(RMSD) [Å]	0.021
結合角の平均二乗偏差(RMSD) [度]	1.727
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Quality characteristics

	Overall
分解能 [Å] (低)	39.650
分解能 [Å] (高)	2.050
Rmerge_l_obs	0.082
独立反射数	86421
完全性 [%]	97.4
冗長性	4.7

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	5	300	5% PEG 4000, 0.1M Sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K