2RJD

Crystal structure of L3MBTL1 protein

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ DW
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-12-10
Detector	RIGAKU RAXIS
Wavelength(s)	1.54000
Spacegroup name	P 21 21 2
格子定数 [Å]	85.518, 94.195, 59.009
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	34.630 - 1.650
R因子	0.20374
Rwork	0.202
R-free	0.23008
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pqw
結合長の平均二乗偏差(RMSD) [Å]	0.012
結合角の平均二乗偏差(RMSD) [度]	1.342
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Quality characteristics

	Overall
分解能 [Å] (低)	34.630
分解能 [Å] (高)	1.650
Rmerge_l_obs	0.057
独立反射数	57382
完全性 [%]	98.5
冗長性	5.7

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	4.6	300	4% PEG 4000, 0.1M Sodium acetate, 1mM H1bK26me2 peptide, 0.1 M Sodium acetate pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K