2RJC

Crystal structure of L3MBTL1 protein in complex with MES

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ DW
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-12-10
Detector	RIGAKU RAXIS
Wavelength(s)	1.54000
Spacegroup name	P 1 21 1
格子定数 [Å]	63.548, 108.784, 90.348
格子定数 [度]	90.00, 90.91, 90.00

精密化法

残基	90.170 - 2.000
R因子	0.19718
Rwork	0.195
R-free	0.24127
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pqw
結合長の平均二乗偏差(RMSD) [Å]	0.020
結合角の平均二乗偏差(RMSD) [度]	1.679
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Quality characteristics

	Overall
分解能 [Å] (低)	90.170
分解能 [Å] (高)	2.000
Rmerge_l_obs	0.088
独立反射数	82054
完全性 [%]	99.9
冗長性	3.6

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	5.1	300	4% PEG 4000, 0.1 M Sodium acetate, 0.1 M MES pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 300K