2RFI
Crystal structure of catalytic domain of human euchromatic histone methyltransferase 1 in complex with SAH and dimethylated H3K9 peptide
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
格子定数 [Å] | 84.593, 85.627, 95.670 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 42.800 - 1.590 |
R因子 | 0.19403 |
Rwork | 0.193 |
R-free | 0.22014 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2igq |
結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
結合角の平均二乗偏差(RMSD) [度] | 1.171 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Quality characteristics
Overall | |
分解能 [Å] (低) | 42.800 |
分解能 [Å] (高) | 1.590 |
Rmerge_l_obs | 0.080 |
独立反射数 | 89676 |
<I/σ(I)> | 10.8 |
完全性 [%] | 96.5 |
冗長性 | 7.4 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 300 | 16% PEG 4000, 10% Isopropanol, 0.1M Bicine pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |