2REX
Crystal structure of the effector domain of PLXNB1 bound with Rnd1 GTPase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-24 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.91790 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 150.136, 71.653, 101.885 |
Unit cell angles | 90.00, 128.36, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.216 |
Rwork | 0.215 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 2CLS 2r2o |
RMSD bond length | 0.017 |
RMSD bond angle | 1.448 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.380 |
High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
Rmerge | 0.060 | 0.053 | 0.231 |
Number of reflections | 36932 | ||
<I/σ(I)> | 9.1 | ||
Completeness [%] | 97.3 | 97.8 | 84.8 |
Redundancy | 3.7 | 3.6 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% PEG 3350, 0.2M Calcium chloride, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |