2R3J

Crystal Structure of Cyclin-Dependent Kinase 2 with inhibitor

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-BM
Synchrotron site	APS
Beamline	17-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2002-10-01
Detector	MARRESEARCH
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
格子定数 [Å]	53.500, 72.070, 72.100
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	27.600 - 1.650
R因子	0.2
Rwork	0.198
R-free	0.23600
Structure solution method	FOURIER SYNTHESIS
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	BUSTER-TNT

Quality characteristics

	Overall	Inner shell	Outer shell
分解能 [Å] (低)	50.000	50.000	1.660
分解能 [Å] (高)	1.650	5.640	1.650
Rmerge_l_obs	0.079	0.060	0.462
独立反射数	27480
<I/σ(I)>	21.7
完全性 [%]	80.8	86.3	57.8

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION	7.4	298	50 mM Na-HEPES pH 7.4, 50 mM Ammonium Acetate, 8% PEG 4000, 4% Glycerol, 1 mM TCEP, vapor diffusion, temperature 298K