2QVO
Crystal structure of AF1382 from Archaeoglobus fulgidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97240, 1.90000 |
| Spacegroup name | P 42 |
| Unit cell lengths | 53.033, 53.033, 40.968 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.500 - 1.850 |
| R-factor | 0.23874 |
| Rwork | 0.237 |
| R-free | 0.27983 |
| Structure solution method | Sulfur SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.121 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SGXPRO |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 19060 | |
| <I/σ(I)> | 12.96 | |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 13.3 | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.6 | 291 | USING 1.0 MICROLITER DROPS CONTAINING EQUAL VOLUMES OF PROTEIN CONCENTRATE (2.8 mg/ml) AND SOLUTION CONTAINING 0.2 M AMMONIUM SULFATE, 0.1 M SODIUM ACETATE, 30% w/v POLYETHYLENE GLYCOL MONOMETHYL ETHER, pH 4.6, MICROBATCH, temperature 291K |
