2QIC

Crystal Structure of the ING1 PHD Finger in complex with a Histone H3K4ME3 peptide

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	2007-02-26
Wavelength(s)	1.257
Spacegroup name	P 32 2 1
格子定数 [Å]	49.060, 49.060, 52.620
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	25.000 - 2.100
Rwork	0.240
R-free	0.26400
Structure solution method	Rigid Body Refinement
Starting model (for MR)	2g6q
結合長の平均二乗偏差(RMSD) [Å]	0.006
結合角の平均二乗偏差(RMSD) [度]	1.091
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	CNS

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	25.000	2.180
分解能 [Å] (高)	2.100	2.100
Rmerge_l_obs	0.071	0.253
独立反射数	4526
<I/σ(I)>	16.3	5.4
完全性 [%]	100.0	100
冗長性	9.35	9.78

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7	277	0.01 M NiCl2 6H20, 0.1 M Tris, 20% polyethylene glycol monomethyl ether 2K, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K