2QGI
The UDP complex structure of the sixth gene product of the F1-ATPase operon of Rhodobacter blasticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.043 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 123.370, 89.928, 69.572 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.990 - 1.650 |
| R-factor | 0.16 |
| Rwork | 0.159 |
| R-free | 0.18400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nxv |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.104 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (CCP4_3.2.17) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 93646 | |
| <I/σ(I)> | 16.7 | 4.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.1 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 100mM sodium citrate (pH6.0) and 75mM magnesium acetate. Mixed 4+4ul with protein sample, hanging drop, vapor diffusion, temperature 298K., VAPOR DIFFUSION, HANGING DROP |
