2Q3F
X-ray crystal structure of putative human Ras-related GTP binding D in complex with GMPPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-14 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97942, 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.730, 67.130, 66.360 |
Unit cell angles | 90.00, 110.92, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.100 |
R-factor | 0.223 |
Rwork | 0.221 |
R-free | 0.26800 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.280 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.120 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.542 | |
Number of reflections | 24803 | |
<I/σ(I)> | 2.4 | |
Completeness [%] | 99.8 | 99.1 |
Redundancy | 4.1 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.4 | 298 | 31% PEG4000, 0.2M sodium acetate and 0.1M Tris HCl, pH 8.4, VAPOR DIFFUSION, temperature 298K |