2PZY
Structure of MK2 Complexed with Compound 76
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-08 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 63 |
| 格子定数 [Å] | 159.610, 159.610, 134.500 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 34.320 - 2.900 |
| Rwork | 0.251 |
| R-free | 0.31300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.154 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 34.320 | 3.000 |
| 分解能 [Å] (高) | 2.900 | 2.900 |
| Rmerge_l_obs | 0.109 | 0.482 |
| Total number of observations | 10846 | |
| 独立反射数 | 42263 | |
| <I/σ(I)> | 8.2 | 1.8 |
| 完全性 [%] | 98.0 | 94.7 |
| 冗長性 | 3 | 2.68 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 297 | 100 mM HEPES pH 7.5, 1.85 M AMMONIUM SULFATE, vapor diffusion, hanging drop, temperature 297K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 297 | 100 mM HEPES pH 7.5, 1.85 M AMMONIUM SULFATE, vapor diffusion, hanging drop, temperature 297K |
