2PW6
Crystal structure of uncharacterized protein JW3007 from Escherichia coli K12
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-06 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97243 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 83.940, 83.940, 85.509 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 72.740 - 2.270 |
R-factor | 0.23616 |
Rwork | 0.235 |
R-free | 0.26670 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.703 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SCA2STRUCTURE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.740 | 2.350 |
High resolution limit [Å] | 2.270 | 2.270 |
Number of reflections | 16342 | |
<I/σ(I)> | 18.1 | 5.63 |
Completeness [%] | 99.6 | 100 |
Redundancy | 20.7 | 20.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 2 microliter drops containing equal volumes of protein concentrate (11.38 mg/ml) in 20mM Tris-HCl, 200mM NaCl, 1mM DTT and resevoir solution containing 0.1M Hepes, 0.8M Sodium acetate, and 0.05M Cadmium sulfate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |