2PQ5
Crystal structure of Dual specificity protein phosphatase 13 (DUSP13)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-16 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.03310 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 73.821, 73.821, 303.803 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.1765 |
Rwork | 0.176 |
R-free | 0.26030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gwo |
RMSD bond length | 0.005 |
RMSD bond angle | 0.022 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.088 | 0.598 |
Number of reflections | 43981 | |
<I/σ(I)> | 15 | 2 |
Completeness [%] | 99.9 | 99.1 |
Redundancy | 5.6 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 25% PEG3350, 0.15 M Ammonium citrate pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |