2PGI
THE CRYSTAL STRUCTURE OF PHOSPHOGLUCOSE ISOMERASE-AN ENZYME WITH AUTOCRINE MOTILITY FACTOR ACTIVITY IN TUMOR CELLS
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 287 |
Detector technology | IMAGE PLATE |
Collection date | 1997-07-25 |
Detector | RIGAKU RAXIS II |
Spacegroup name | I 2 2 2 |
格子定数 [Å] | 75.100, 93.730, 171.900 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 8.000 - 2.300 |
R因子 | 0.185 |
Rwork | 0.185 |
R-free | 0.25800 * |
Structure solution method | MIRAS |
結合長の平均二乗偏差(RMSD) [Å] | 0.012 |
結合角の平均二乗偏差(RMSD) [度] | 26.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 30.700 | 2.380 |
分解能 [Å] (高) | 2.300 | 2.300 |
Rmerge_l_obs | 0.070 | 0.362 * |
Total number of observations | 104060 * | |
独立反射数 | 24024 | |
<I/σ(I)> | 12 | 3.2 |
完全性 [%] | 93.9 | 83.4 |
冗長性 | 4.3 | 3.1 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | Vapor diffusion, hanging drop * | 7 | pH 7.0 |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
1 | 1 | drop | protein | (mg/ml) | |
2 | 1 | drop | 6-phosphogluconate | (mM) | |
3 | 1 | drop | phosphate | (mM) | |
4 | 1 | reservoir | phosphate | (mM) | |
5 | 1 | reservoir | ammonium phosphate | (M) |