2P9M
Crystal structure of conserved hypothetical protein MJ0922 from Methanocaldococcus jannaschii DSM 2661
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-31 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97240 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 57.207, 94.688, 102.042 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 36.470 - 2.590 |
| R因子 | 0.22544 |
| Rwork | 0.222 |
| R-free | 0.29177 |
| Structure solution method | SAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.013 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.435 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SGXPRO |
| Refinement software | REFMAC (5.2.0019) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.690 |
| 分解能 [Å] (高) | 2.590 | 2.590 |
| 独立反射数 | 17759 | |
| <I/σ(I)> | 16.7 | 4.73 |
| 完全性 [%] | 99.4 | 96.3 |
| 冗長性 | 12.6 | 9.3 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | MICROBATCH UNDER OIL | 6.3 | 289 | USING 1.0 MICROLITER DROPS CONTAINING EQUAL VOLUMES OF PROTEIN CONCENTRATE (27.49 mg/ml) AND SOLUTION CONTAINING 27.5% w/v PEG 4000, 0.1 M MES-SODIUM HYDROXIDE, pH 6.3, MICROBATCH UNDER OIL, temperature 289K |
