2P0A
The crystal structure of human synapsin III (SYN3) in complex with AMPPNP
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.99991 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 130.796, 73.186, 78.398 |
| 格子定数 [度] | 90.00, 100.74, 90.00 |
精密化法
| 残基 | 47.000 - 1.900 |
| R因子 | 0.18311 |
| Rwork | 0.181 |
| R-free | 0.21804 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Swissmodel based on PDB entries: 1I7L 1i7n 1pk8 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.012 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.358 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 64.280 | 2.000 |
| 分解能 [Å] (高) | 1.900 | 1.900 |
| Rmerge_l_obs | 0.132 | 0.477 |
| 独立反射数 | 56940 | |
| 完全性 [%] | 99.2 | 94.4 |
| 冗長性 | 3.5 | 2.3 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 1.26M (NH4)2SO4, 0.2M Li2SO4, Tris-HCl pH 8.5, 20 % Ethylene glycol added as cryoprotectant, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
