2OY0

Crystal structure of the West Nile virus methyltransferase

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-13
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00925
Spacegroup name	P 1
格子定数 [Å]	39.229, 66.009, 78.761
格子定数 [度]	112.46, 102.90, 89.80

精密化法

残基	39.120 - 2.800
R因子	0.259
Rwork	0.259
R-free	0.33400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l9k
結合長の平均二乗偏差(RMSD) [Å]	0.008
結合角の平均二乗偏差(RMSD) [度]	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS (1.0)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	39.120	2.960
分解能 [Å] (高)	2.800	2.800
Rmerge_l_obs	0.112	0.214
独立反射数	15957
<I/σ(I)>	4.3	2.5
完全性 [%]	0.9	0.917
冗長性	1.7	1.6

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	13 to 15% PEG4000, 5% isopropanol, 0.1M sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K