2OX7
Crystal structure of protein EF1440 from Enterococcus faecalis
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97961 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 64.346, 70.376, 150.914 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 19.630 - 1.777 |
| R因子 | 0.19208 |
| Rwork | 0.190 |
| R-free | 0.23531 |
| Structure solution method | SAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.017 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.532 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 25.474 | 25.470 | 1.870 |
| 分解能 [Å] (高) | 1.777 | 5.620 | 1.777 |
| Rmerge_l_obs | 0.080 | 0.055 | 0.383 |
| Total number of observations | 30071 | 116843 | |
| 独立反射数 | 66447 | ||
| <I/σ(I)> | 6.2 | 11.2 | 2 |
| 完全性 [%] | 99.4 | 98.5 | 96.1 |
| 冗長性 | 14.4 | 13.1 | 12.6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 2.0M SODIUM CHLORIDE, 10% PEG 6000, pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
