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2OKK

The X-ray crystal structure of the 65kDa isoform of Glutamic Acid Decarboxylase (GAD65)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 17-ID
Synchrotron siteAPS
Beamline17-ID
Temperature [K]100
Detector technologyCCD
Collection date2006-04-01
DetectorADSC QUANTUM 4
Wavelength(s)1.000
Spacegroup nameC 2 2 21
Unit cell lengths78.251, 99.057, 120.009
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution37.190 - 2.300
R-factor0.2
Rwork0.196
R-free0.25600
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2okj
RMSD bond length0.009
RMSD bond angle1.352
Data reduction softwareHKL-2000
Data scaling softwareSCALA (CCP4_3.2.17 23/9/2005)
Phasing softwarePHASER
Refinement softwareREFMAC (5.2.0019)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]37.19054.6402.420
High resolution limit [Å]2.3007.2702.300
Total number of observations224711442
Number of reflections20717
Completeness [%]91.8698.85
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.229320% ethanol, 100 mM MES, 10 mM 2-mercaptoethanol, 20 mM CaCl2, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K

217705

数据于2024-03-27公开中

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