2OHA
Myoglobin cavity mutant F138W
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-03-15 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 6 |
格子定数 [Å] | 90.180, 90.180, 45.340 |
格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
残基 | 29.590 - 1.800 |
Rwork | 0.166 |
R-free | 0.20000 |
Structure solution method | MOLECULAR REPLACEMENT |
結合長の平均二乗偏差(RMSD) [Å] | 0.012 |
結合角の平均二乗偏差(RMSD) [度] | 1.800 |
Data scaling software | d*TREK |
Refinement software | CNS (1.1) |
Quality characteristics
Overall | Inner shell | Outer shell | |
分解能 [Å] (低) | 29.590 | 29.590 | 1.590 |
分解能 [Å] (高) | 1.540 | 3.310 | 1.540 |
Rmerge_l_obs | 0.048 | 0.037 | 0.144 |
Total number of observations | 10996 | 649 | |
独立反射数 | 26942 | ||
<I/σ(I)> | 16 | 31.4 | 2.6 |
完全性 [%] | 85.4 | 99.7 | 19.4 |
冗長性 | 2.89 | 3.32 | 1.08 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | PROTEIN SOLUTION (20 MG/ML PROTEIN, 0.020 M TRIS-HCL, pH 9.0), MIXED IN A 1:1 RATIO WITH THE WELL SOLUTION (2.8 M AMMONIUM SULPHATE, 0.050 M TRIS-HCL, pH 9.0), cryo-protected with 20% SUCROSE, vapor diffusion, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP |