2O8M

Crystal structure of the S139A mutant of Hepatitis C Virus NS3/4A protease

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	200
Detector technology	IMAGE PLATE
Collection date	1997-08-01
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	H 3 2
格子定数 [Å]	224.640, 224.640, 75.360
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	50.000 - 2.000
Rwork	0.194
R-free	0.22800
Structure solution method	FOURIER SYNTHESIS
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BUSTER
Refinement software	BUSTER

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.030
分解能 [Å] (高)	2.000	2.000
Rmerge_l_obs	0.042	0.572
独立反射数	46014
<I/σ(I)>	19.4	1.1
完全性 [%]	94.1	58.7
冗長性	3	1.4

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP		277	1.25 to 1.5M NaCl, 0.1 M MES, .1M Na/K PO4, 5mM beta-mercaptoethanol, pH 5.6-6.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K