2O8J
Human euchromatic histone methyltransferase 2
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| 格子定数 [Å] | 56.586, 70.965, 83.046 |
| 格子定数 [度] | 81.11, 75.13, 88.39 |
精密化法
| 残基 | 79.310 - 1.800 |
| R因子 | 0.18385 |
| Rwork | 0.182 |
| R-free | 0.22124 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2igq |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.013 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.347 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 79.300 |
| 分解能 [Å] (高) | 1.800 |
| Rmerge_l_obs | 0.048 |
| 独立反射数 | 100323 |
| <I/σ(I)> | 13.7 |
| 完全性 [%] | 92.2 |
| 冗長性 | 3.2 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | peg 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
