2O7M
The C-terminal loop of the homing endonuclease I-CreI is essential for DNA binding and cleavage. Identification of a novel site for specificity engineering in the I-CreI scaffold
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97 |
Spacegroup name | P 43 |
Unit cell lengths | 69.088, 69.088, 93.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.540 - 2.000 |
R-factor | 0.18634 |
Rwork | 0.184 |
R-free | 0.23464 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g9z |
RMSD bond length | 0.029 |
RMSD bond angle | 2.137 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.000 |
Number of reflections | 25943 |
Completeness [%] | 93.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |