2O07

Human spermidine synthase

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-03-10
Detector	RIGAKU
Wavelength(s)	1.54178
Spacegroup name	P 1 21 1
格子定数 [Å]	58.125, 60.663, 86.705
格子定数 [度]	90.00, 108.68, 90.00

精密化法

残基	82.200 - 1.890
R因子	0.17906
Rwork	0.176
R-free	0.22751
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.017
結合角の平均二乗偏差(RMSD) [度]	1.562
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Quality characteristics

	Overall
分解能 [Å] (低)	82.200
分解能 [Å] (高)	1.890
Rmerge_l_obs	0.088
独立反射数	44511
完全性 [%]	97.9
冗長性	4.4

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7	300	20% PEG 3350, 0.2M Mg(OAc)2, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K