2MEG
CHANGES IN CONFORMATIONAL STABILITY OF A SERIES OF MUTANT HUMAN LYSOZYMES AT CONSTANT POSITIONS.
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-12-12 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 57.100, 61.060, 33.760 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 8.000 - 1.800 |
| R因子 | 0.151 |
| Rwork | 0.151 |
| Structure solution method | ISOMORPHOUS METHOD |
| Starting model (for MR) | WILD-TYPE OF HUMAN LYSOZYME |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.008 |
| 結合角の平均二乗偏差(RMSD) [度] | 23.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 1.830 | |
| 分解能 [Å] (高) | 1.800 | 1.800 |
| Rmerge_l_obs | 0.052 | 0.180 |
| Total number of observations | 42094 * | |
| 独立反射数 | 10582 * | |
| <I/σ(I)> | 8.8 | |
| 完全性 [%] | 92.2 | 80.9 |
| 冗長性 | 2.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, hanging drop * | 4.5 | 10 * | Takano, K., (1995) J.Mol.Biol., 254, 62. * |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | 2.5 (M) | ||
| 3 | 1 | reservoir | acetate | 20 (mM) |
