2JLN

Structure of Mhp1, a nucleobase-cation-symport-1 family transporter

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-10
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
格子定数 [Å]	79.700, 109.140, 113.820
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	29.014 - 2.850
R因子	0.2362
Rwork	0.234
R-free	0.28090
Structure solution method	MIRAS
Starting model (for MR)	NONE
結合長の平均二乗偏差(RMSD) [Å]	0.010
結合角の平均二乗偏差(RMSD) [度]	1.480
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	PHENIX ((PHENIX.REFINE))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	30.000	2.950
分解能 [Å] (高)	2.850	2.850
Rmerge_l_obs	0.120	0.770
独立反射数	22414
<I/σ(I)>	7.2	1
完全性 [%]	94.0	81
冗長性	2.9	2.3

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1		7		PROTEIN WAS CRYSTALLIZED FROM 31-33 % PEG 300, 100 MM NACL AND 100 MM NA-PHOSPHATE (PH 7.0), THAN SOAKED FOR 1 MINUTE IN 2.5 MM METHYL MERCURY ACETATE.