2JH7
Crystal structure of Toxoplasma gondii micronemal protein 1 bound to 6'-sialyl-N-acetyllactosamine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-09-29 |
Detector | ADSC CCD |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 65.935, 65.935, 172.983 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.290 - 2.070 |
R-factor | 0.184 |
Rwork | 0.183 |
R-free | 0.21200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jh1 |
RMSD bond length | 0.021 |
RMSD bond angle | 1.794 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 49275 | |
<I/σ(I)> | 43.5 | 6.7 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 7.6 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | VAPOUR DIFFUSION IN HANGING DROP 3.6M AMMONIUM ACETATE 100 MM BIS-TRIS-PROPANE PH 7.8 |